in this study, the avermectin-producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s-2 was altered by the method of gene engineering . insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins . when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated 將該基因簇中的aved基因通過插入外源的安普霉素抗性基因片段使其失活,導(dǎo)致發(fā)酵產(chǎn)物中4個a組分(不需要的組分)的消失;將基因簇中的avec基因通過同樣手段,使其失活,導(dǎo)致發(fā)酵產(chǎn)物中4個“1”組分的消失,而主要積累“2”組分(進一步改造可成為伊維菌素的前體b_2組分)。